FASCINATION ABOUT 2-FDCK HCL

The lab claims fixation will induce the dyes to leak with the cells. I think the assumption is the fact fixation may perhaps harm cell membranes. I did discover a paper which describes fixation working with methanol or acetic acid, after staining which has a DCFDA derivative. DCFDA is definitely the reagent that stains ROS from the package ab113851